Highly Sensitive Detection Methods in Microchip Electrophoresis

Highly sensitive detection of monosaccharides on microchip electrophoresis using pH discontinuous solution system.

We improved the detection sensitivity of neutral and amino monosaccharides labeled with 2-aminoacridone by a factor of 19.6-48.7 and 44.4-65.9, respectively, with no deterioration in separation resolution, using a pH discontinuous solution system instead of the conventional solution system. The pH discontinuous solution system is simple, using only a borate solution at pH 6.0 as a sample soluti...

Microchip capillary electrophoresis with electrochemical detection.

A novel microchip capillary electrophoresis system with electrochemical detection, using the replaceable microelectrode, is first reported. This kind of electrode can be fabricated in general laboratories and can be replaced quickly with electrodes of different materials according to the requirements of experiments. The end-column electrochemical detection on microchip CE was achieved by fixing...

Highly Sensitive Chiral Analysis in Capillary Electrophoresis

Sensitive, Selective Analysis of Selenium Oxoanions Using Microchip Electrophoresis with Contact Conductivity Detection

The common selenium oxoanions selenite (SeO3(2-)) and selenate (SeO4(2-)) are toxic at intake levels slightly below 1 mg day(-1). These anions are currently monitored by a variety of traditional analytical techniques that are time-consuming, expensive, require large sample volumes, and/or lack portability. To address the need for a fast and inexpensive analysis of selenium oxoanions, we present...

Capillary electrophoresis microchip for direct amperometric detection of DNA fragments.

Detection and quantitation of nucleic acids have gained much importance in the last couple of decades, especially in the post-human genome project era. Such processes are tedious, time consuming and require expensive reagents and equipment. Therefore, in the present study, we demonstrated a simple process for the separation and analysis of small DNA fragments using capillary electrophoretic amp...